Neuronal cell culture stained with DAPI, beta III Tubulin–Cy2, Nestin-Cy3 (LMS Bioanalytik GmbH, Magdeburg, Germany). Blue indicates the nuclei of the cells, green neurons expressing beta III Tubulin, and red stem cells expressing Nestin. Image was acquired with a M205 FCA stereo microscope, LMT260 x/y stage, DFC3000 G microscope camera and Fluocombi III at 400x.
Zebrafish, mpx:eGFP line: time lapse images of the neutrophils to the tail resection. Courtesy of Dr Carl Tucker, The Queen’s Medical Research Institute, University Edinburgh, United Kingdom. In this in vivo model the inflammatory response in a transgenic zebrafish line that expresses GFP under the neutrophil-specific myeloperoxidase promoter is shown. “Quantitative data can be generated from this model by counting of fluorescent cells or by digital image analysis” (Renshaw et al Blood 2006) DOI 10.1182/blood-2006-05-024075
Fluorescence video imaging of a zebrafish larva expressing green fluorescent protein (GFP) immersed in an aqueous solution. The images were captured with a Leica M165 FC stereo microscope where a Leica 2x Plan Apo objective with (right image) and without (left image) correction collar was used. The heart of the zebrafish larva is seen beating in both videos. The larva appears blurrier in the non-corrected image on the right. Courtesy of M. J. Hamm and W. Herzog, Angiogenesis Laboratory, Max Planck Institute for Molecular Biomedicine and Westfälische Wilhelms University in Münster, Germany. Solea senegalensis larvae nervous system, max projection of a tile scan of 6 fields x 33 planes. Parallax correction and tiling performed in LAS X after deconvolution with Huygens professional. Courtesy of Dr. Marco A. Campinho, CCMAR - Centre for Marine Sciences, Universidade do Algarve, Portugal.
探索 LAS X 软件在生命科学中的应用
LAS X 是所有徕卡显微镜解决方案的软件平台。让运行复杂荧光实验变得简单。LAS X (注意：新 LSR 用 LAS X 的网页链接) 将指导您逐步完成整个分析工作流程。
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